The chromatography adsorbents are generally used to separate the mixture of various substances that are available in very small quantity into the mixture. When a compound is mixed with different components that have same chemical and physical properties, it gets tougher to separate the component from the mixture. Common adsorbents don’t perform significantly in such cases.
The process works by distributing the mixture into two different phases namely- fixed phase and the mobile phase. Where the fixed phase can be the column of adsorbent, paper or thin layer of adsorbing material on a glass plate where the unwanted components are absorbed by the plate but, on the contrary, the compounds used in mobile phase pass through quite easily. Thus chromatography helps in producing pure liquids.
Various types of adsorbents are used in chromatography process which is based on the type of mixtures of components that are about to be separated such as; silica gel, aluminum oxide, thin layer, flash and column chromatography, etc. and it is important to select the perfect adsorbent for the mixtures.
This form of chromatography is also called the medium pressure chromatography which is generally performed to separate a mixture of two solvents between which one is polar component and the other one is non-polar component. This chromatographic separation process is actually works by external air flow. The solvent is flown down to the column with the help of this air flow. The process is carried out to separate the organic compound from the mixture of it with other unwanted compounds and impurities as well. In such chromatography, the retention factor plays the major role to decide which solvent would be appropriate for this event. The percentage of desired natural product produced from this process is really higher from the general expectation and compared to other conventional column chromatography. This is because of the use of high quality silica gel 230-400 mesh and silica gel 200-400 mesh.
Normally, the smaller particles which contain a higher mesh value are used in flash column chromatography because; it is believed to be the simplest process of separating the sample and the solvent. When it comes to purifying the organic compounds, this process acts quite faster and in a cost-effective way and furthermore, with a better outcome of the expected compounds as well. The benefits of flash chromatography include less usage of the solvent, greater flexibility, quicker separation capacity and a higher rate of reproducibility as well.